Mechanomyographic Amplitude-Force Relationships of the Biceps Brachii Differ Between Sexes During Linearly Varying Muscle Actions and Provide Insight on Muscle Morphology
DOI:
https://doi.org/10.63750/9j1ghf75Keywords:
Biceps brachii, Log-transform model, Mechanomyography, Motor unit activation/deactivation, UltrasonographyAbstract
This study compared mechanomyographic amplitude (MMGRMS)-force relationships of the biceps brachii (BB) between sexes during moderate- and high-intensity increasing, steady, and decreasing isometric motor tasks. Twenty participants (ten males, ten females) performed maximal voluntary contractions (MVCs) of the elbow flexors followed by isometric trapezoidal muscle actions at 40% and 70% MVC. MMG signals were recorded from the BB. MMGRMS-force relationships during the increasing and decreasing segments were log-transformed to calculate a (gain) and b (slope) terms. MMGRMS was averaged during steady force. BB muscle cross-sectional area (mCSA) was measured via ultrasonography. The b terms, collapsed across segment and intensity, were greater for males (0.665±0.160) than the females (0.360±0.184; p<0.001). The a terms for the females during the 40% MVC (0.103±0.101) were greater than the males (0.018±0.014; p=0.026). MMGRMS, collapsed across intensity, was greater in males (0.256±0.091 m·s-2) than females (0.159±0.041 m·s-2; p=0.006). mCSA was correlated with the b terms (p=0.001; r=0.67) and MMGRMS (p<0.001; r=0.75). The greater b terms for the males and the relationships between b terms and mCSA may result from larger MUs producing greater twitch forces throughout the recruitment spectrum. MMGRMS differentiated BB MU behavior between sexes during various muscle actions and was sensitive to muscle size.
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Akataki, K., Mita, K., Watakabe, M., & Itoh, K. (2001). Mechanomyogram and force relationship during voluntary isometric ramp contractions of the biceps brachii muscle. European Journal of Applied Physiology, 84(1), 19–25. https://doi.org/10.1007/s004210000321
Akataki, K., Mita, K., Watakabe, M., & Itoh, K. (2003). Mechanomyographic responses during voluntary ramp contractions of the human first dorsal interosseous muscle. European Journal of Applied Physiology, 89(6), 520–525. https://doi.org/10.1007/s00421-003-0835-1
Beck, T., Housh, T., Johnson, G., Weir, J., Cramer, J., Coburn, J., & Malek, M. (2004). Mechanomyographic amplitude and mean power frequency versus torque relationships during isokinetic and isometric muscle actions of the biceps brachii. Journal of Eletromyography and Kinesiology, 14(5), 555–564.https://doi.org/10.1016/j.jelekin.2004.03.001
Beck, T. W., DeFreitas, J. M., Stock, M. S., & Dillon, M. A. (2010). An examination of mechanomyographic signal stationarity during concentric isokinetic, eccentric isokinetic and isometric muscle actions. Physiological Measurement, 31(3), 339. https://doi.org/10.1088/0967-3334/31/3/005
Berg, H., Tedber, B., & Tesch, P. (1993). Changes in lower limb muscle cross-sectional area and tissue fluid volume after transition from standing to supine. Acta Physiologica Scandinavica, 148(4), 379–385. https://doi.org/10.1111/j.1748-1716.1993.tb09573.x
Bichler, E. (2000). Mechanomyograms recorded during evoked contractions of single motor units in the rat medial gastrocnemius muscle. European Journal of Applied Physiology, 83(4), 310–319. https://doi.org/10.1007/s004210000261
Coburn, J. W., Housh, T. J., Cramer, J. T., Weir, J. P., Miller, J. M., Beck, T. W., Malek, M. H., & Johnson, G. O. (2004). Mechanomyographic time and frequency domain responses of the vastus medialis muscle during submaximal to maximal isometric and isokinetic muscle actions. Electromyography and Clinical Neurophysiology, 44(4), 247–255.
Coburn, J. W., Housh, T. J., Cramer, J. T., Weir, J. P., Miller, J. M., Beck, T. W., Malek, M. H., & Johnson, G. O. (2005). MECHANOMYOGRAPHIC AND ELECTROMYOGRAPHIC RESPONSES OF THE VASTUS MEDIALIS MUSCLE DURING ISOMETRIC AND CONCENTRIC MUSCLE ACTIONS. The Journal of Strength & Conditioning Research, 19(2), 412.
Cooper, M. A., & Herda, T. J. (2014). Muscle-related differences in mechanomyography–force relationships are model-dependent. Muscle & Nerve, 49(2), 202–208. https://doi.org/10.1002/mus.23896
Dalton, P. A., & Stokes, M. J. (1991). Acoustic myography reflects force changes during dynamic concentric and eccentric contractions of the human biceps brachii muscle. European Journal of Applied Physiology and Occupational Physiology, 63(6), 412–416. https://doi.org/10.1007/BF00868071
De Luca, C. J., & Hostage, E. C. (2010). Relationship Between Firing Rate and Recruitment Threshold of Motoneurons in Voluntary Isometric Contractions. Journal of Neurophysiology, 104(2), 1034–1046. https://doi.org/10.1152/jn.01018.2009
De Luca, C. J., LeFever, R. S., McCue, M. P., & Xenakis, A. P. (1982). Behaviour of human motor units in different muscles during linearly varying contractions. The Journal of Physiology, 329(1), 113–128. https://doi.org/10.1113/jphysiol.1982.sp014293
Dimmick, H., Miller, J., Sterczala, A., Trevino, M., & Herda, T. (2018). Vastus lateralis muscle tissue composition and motor unit properties in chronically endurance-trained vs. Sedentary women. European Journal of Applied Physiology, 118, 1789–1800. https://doi.org/10.1007/s00421-018-3909-9
Farina, D., Holobar, A., Merletti, R., & Enoka, R. M. (2010). Decoding the neural drive to muscles from the surface electromyogram. Clinical Neurophysiology, 121(10), 1616–1623. https://doi.org/10.1016/j.clinph.2009.10.040
Farina, D., Merletti, R., & Enoka, R. M. (2004). The extraction of neural strategies from the surface EMG. Journal of Applied Physiology, 96(4), 1486–1495. https://doi.org/10.1152/japplphysiol.01070.2003
Garcia, M. A. C., Vargas, C. D., De Souza, M. N., Imbiriba, L. A., & De Oliveira, L. F. (2008). Evaluation of Arm Dominance by Using the Mechanomyographic Signal. Journal of Motor Behavior, 40(2), 83–89. https://doi.org/10.3200/JMBR.40.2.83-89
Gillen, Z. M., Shoemaker, M. E., McKay, B. D., Bohannon, N. A., Gibson, S. M., & Cramer, J. T. (2019). Muscle strength, size, and neuromuscular function before and during adolescence. European Journal of Applied Physiology, 119(7), 1619–1632. https://doi.org/10.1007/s00421-019-04151-4
Guo, J.-Y., Zheng, Y.-P., Xie, H.-B., & Chen, X. (2010). Continuous monitoring of electromyography (EMG), mechanomyography (MMG), sonomyography (SMG) and torque output during ramp and step isometric contractions. Medical Engineering & Physics, 32(9), 1032–1042. https://doi.org/10.1016/j.medengphy.2010.07.004
Harwood, B., & Rice, C. L. (2014). Short interspike intervals and double discharges of anconeus motor unit action potentials for the production of dynamic elbow extensions. Journal of Neurophysiology, 111(10), 2039–2046. https://doi.org/10.1152/jn.00412.2013
Herda, T. J., Housh, T. J., Fry, A. C., Weir, J. P., Schilling, B. K., Ryan, E. D., & Cramer, J. T. (2010). A noninvasive, log-transform method for fiber type discrimination using mechanomyography. Journal of Electromyography and Kinesiology, 20(5), 787–794. https://doi.org/10.1016/j.jelekin.2010.01.004
Herda, T. J., Ryan, E. D., Kohlmeier, M., Trevino, M. A., Gerstner, G. R., & Roelofs, E. J. (2018). Examination of muscle morphology and neuromuscular function in normal weight and overfat children aged 7-10 years. Scandinavian Journal of Medicine & Science in Sports, 28(11), 2310–2321. https://doi.org/10.1111/sms.13256
Herda, T. J., Siedlik, J. A., Trevino, M. A., Cooper, M. A., & Weir, J. P. (2015). Motor unit control strategies of endurance- versus resistance-trained individuals. Muscle & Nerve, 52(5), 832–843. https://doi.org/10.1002/mus.24597
Herda, T. J., Trevino, M. A., Sterczala, A. J., Miller, J. D., Wray, M. E., Dimmick, H. L., Gallagher, P. M., & Fry, A. C. (2019). Muscular strength and power are correlated with motor unit action potential amplitudes, but not myosin heavy chain isoforms in sedentary males and females. Journal of Biomechanics, 86, 251–255. https://doi.org/10.1016/j.jbiomech.2019.01.050
Herda, T. J., Weir, J. P., Ryan, E. D., Walter, A. A., Costa, P. B., Hoge, K. M., Beck, T. W., Stout, J. R., & Cramer, J. T. (2009). Reliability of absolute versus log-transformed regression models for examining the torque-related patterns of response for mechanomyographic amplitude. Journal of Neuroscience Methods, 179(2), 240–246. https://doi.org/10.1016/j.jneumeth.2009.01.028
Hill, E. C., Housh, T. J., Smith, C. M., Schmidt, R. J., & Johnson, G. O. (2018). Gender- and Muscle-Specific Responses During Fatiguing Exercise. The Journal of Strength & Conditioning Research, 32(5), 1471. https://doi.org/10.1519/JSC.0000000000001996
Jesunathadas, M., Marmon, A. R., Gibb, J. M., & Enoka, R. M. (2010). Recruitment and derecruitment characteristics of motor units in a hand muscle of young and old adults. Journal of Applied Physiology, 108(6), 1659–1667. https://doi.org/10.1152/japplphysiol.00807.2009
Kidgell, D., Stokes, M., Castricum, T., & Pearce, A. (2010). Neurophysiological responses after short-term strength training of the biceps brachii muscle. The Journal of Strength and Conditioning Research, 24(11), 3123–3132. https://doi.org/10.1519/JSC.0b013e3181f56794
Luca, C. J. D., & Kline, J. C. (2011). Influence of proprioceptive feedback on the firing rate and recruitment of motoneurons. Journal of Neural Engineering, 9(1), 016007. https://doi.org/10.1088/1741-2560/9/1/016007
Lulic-Kuryllo, T., & Inglis, J. G. (2022). Sex differences in motor unit behaviour: A review. Journal of Electromyography and Kinesiology, 66, 102689. https://doi.org/10.1016/j.jelekin.2022.102689
Miller, A. E. J., MacDougall, J. D., Tarnopolsky, M. A., & Sale, D. G. (1993). Gender differences in strength and muscle fiber characteristics. European Journal of Applied Physiology and Occupational Physiology, 66(3), 254–262. https://doi.org/10.1007/BF00235103
Miller, J. D., Sterczala, A. J., Trevino, M. A., & Herda, T. J. (2018). Examination of muscle composition and motor unit behavior of the first dorsal interosseous of normal and overweight children. Journal of Neurophysiology, 119(5), 1902–1911. https://doi.org/10.1152/jn.00675.2017
Nonaka, H., Mita, K., Akataki, K., Watakabe, M., & Itoh, Y. (2006). Sex differences in mechanomyographic responses to voluntary isometric contractions. Medicine and Science in Sports and Exercise, 38(7), 1311–1316. https://doi.org/10.1249/01.mss.0000227317.31470.16
Olmos, A. A., Montgomery, T. R., Sears, K. N., Dinyer, T. K., Hammer, S. M., Bergstrom, H. C., Hill, E. C., Succi, P. J., Lawson, J., & Trevino, M. A. (2024). Blood flow restriction increases necessary muscle excitation of the elbow flexors during a single high-load contraction. European Journal of Applied Physiology. https://doi.org/10.1007/s00421-023-05405-y
Olmos, A. A., Sterczala, A. J., Parra, M. E., Dimmick, H. L., Miller, J. D., Deckert, J. A., Sontag, S. A., Gallagher, P. M., Fry, A. C., Herda, T. J., & Trevino, M. A. (2023). Sex-related differences in motor unit behavior are influenced by myosin heavy chain during high- but not moderate-intensity contractions. Acta Physiologica, 239(1), e14024.https://doi.org/10.1111/apha.14024
Olmos, A., Herda, T., Sontag, S., & Trevino, M. (2022). The influence of chronic training status on the mechanical behavior of the vastus lateralis during repetitive trapezidal contractions. Journal of Musculoskeletal and Neuronal Interactions, 22(2), 161–171.
Orizio, C., Perini, R., Diemont, B., Maranzana Figini, M., & Veicsteinas, A. (1990). Spectral analysis of muscular sound during isometric contraction of biceps brachii. Journal of Applied Physiology, 68(2), 508–512. https://doi.org/10.1152/jappl.1990.68.2.508
Orizio, C., Perini, R., & Veicsteinas, A. (1989). Muscular sound and force relationship during isometric contraction in man. European Journal of Applied Physiology and Occupational Physiology, 58(5), 528–533. https://doi.org/10.1007/BF02330708
Parra, M. E., Miller, J. D., Sterczala, A. J., Trevino, M. A., Dimmick, H. L., & Herda, T. J. (2020). Differences in the firing rate versus recruitment threshold relationships of the vastus lateralis in children ages 7–10 years and adults. Human Movement Science, 72, 102650. https://doi.org/10.1016/j.humov.2020.102650
Perez, S., Olmos, A. A., Sontag, S. A., Parra, M. E., Dimmick, H. L., Sterczala, A. J., Miller, J. D., Deckert, J. A., Herda, T. J., & Trevino, M. A. (2022). Effects of Endurance Cycling on Mechanomyographic Median Power Frequency of the Vastus Lateralis. Applied Sciences, 12(10), Article 10. https://doi.org/10.3390/app12105213
Petersen, N., Taylor, J., & Gandevia, S. (2002). The effect of eletrical stimulation of the corticospinal tract on motor units of the human biceps brachii. The Journal of Physiology, 544(1), 277–284. https://doi.org/10.1113/jphysiol.2002.024539
Ryan, E. D., Beck, T. W., Herda, T. J., Hartman, M. J., Stout, J. R., Housh, T. J., & Cramer, J. T. (2008). Mechanomyographic amplitude and mean power frequency responses during isometric ramp vs. Step muscle actions. Journal of Neuroscience Methods, 168(2), 293–305. https://doi.org/10.1016/j.jneumeth.2007.10.010
Sale, D. G., MacDougall, J. D., Alway, S. E., & Sutton, J. R. (1987). Voluntary strength and muscle characteristics in untrained men and women and male bodybuilders. Journal of Applied Physiology, 62(5), 1786–1793. https://doi.org/10.1152/jappl.1987.62.5.1786
Sontag, S. A., Sterczala, A. J., Miller, J. D., Deckert, J. A., Olmos, A. A., Parra, M. E., Dimmick, H. L., Gallagher, P. M., Fry, A. C., Herda, T. J., & Trevino, M. A. (2023). A noninvasive test for estimating myosin heavy chain of the vastus lateralis in females with mechanomyography. Medical Engineering & Physics, 111, 103946. https://doi.org/10.1016/j.medengphy.2022.103946
Sontag, S., Trevino, M., Herda, T., Sterczala, A., Miller, J., Parra, M., & Deckert, J. (2021). Endurance training alters motor unit activation strategies for the vastus lateralis, yet sex-related differences and relationships with muscle size remain. European Journal of Applied Physiology, 121, 1367–1377.
Sterczala, A. J., Herda, T. J., Miller, J. D., Ciccone, A. B., & Trevino, M. A. (2018). Age-related differences in the motor unit action potential size in relation to recruitment threshold. Clinical Physiology and Functional Imaging, 38(4), 610–616. https://doi.org/10.1111/cpf.12453
Trevino, M. A., Herda, T. J., Fry, A. C., Gallagher, P. M., Vardiman, J. P., Mosier, E. M., & Miller, J. D. (2016). The influence of myosin heavy chain isoform content on mechanical behavior of the vastus lateralis in vivo. Journal of Electromyography and Kinesiology, 28, 143–151. https://doi.org/10.1016/j.jelekin.2016.04.005
Trevino, M., Dimmick, H., Parra, M., Sterczala, A., Miller, J., Deckert, J., & Herda, T. (2022). Effects of continuous cycling training on motor unit firing rates, input excitation, and myosin heavy chain of the vastus lateralis in sedentary females. Experimental Brain Research, 240(3), 825–839. https://doi.org/10.1007/s00221-021-06278-3
Trevino, M., & Herda, T. (2015). The effects of chronic exercise training status on motor unit activation and deactivation control strategies. Journal of Sports Sciences, 34(3), 199–208. https://doi.org/10.1080/02640414.2015.1046396
Trevino, M., Perez, S., Sontag, S., Olmos, A., Jeon, S., & Richardson, L. (2023). Influence of Pennation Angle and Muscle Thickness on Mechanomyographic Amplitude–Torque Relationships and Sex-Related Differences in the Vastus Lateralis. Journal of Functional Morphology and Kinesiology, 8(2), Article 2. https://doi.org/10.3390/jfmk8020053
Ye, X., Beck, T., DeFreitas, J., & Wages, N. (2015). Acute effects of dynamic exercises on the relationship between the motor unit firing rate and the recruitment threshold.Human Movement Science, 40, 24–37. https://doi.org/10.1016/j.humov.2014.11.011
Young, H.-J., Jenkins, N. T., Zhao, Q., & Mccully, K. K. (2015). Measurement of intramuscular fat by muscle echo intensity. Muscle & Nerve, 52(6), 963–971. https://doi.org/10.1002/mus.24656
Zwarts, M. J., & Keidel, M. (1991). Relationship between electrical and vibratory output of muscle during voluntary contraction and fatigue. Muscle & Nerve, 14(8), 756–761. https://doi.org/10.1002/mus.880140810
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Copyright (c) 2025 Brenden Roth, Alex Olmos, Kylie Sears, Tony Montgomery, Taylor Dinyer-McNeely, Shane Hammer, Haley Bergstrom, Ethan Hill, Pasquale Succi, Lyric Richardson, Sean Lubiak, Michael Trevino (Author)
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